Optimization in Drug Discovery: In Vitro Methods by Gary W. Caldwell, Zhengyin Yan

By Gary W. Caldwell, Zhengyin Yan

Thoroughly revised and up-to-date, Optimization in Drug Discovery: In Vitro equipment, moment Edition offers a large spectrum of in vitro assays together with formula, plasma binding, absorption and permeability, cytochrome P450 (CYP) and UDP-glucuronosyltransferases (UGT) metabolism, CYP inhibition and induction, drug transporters, drug-drug interactions through review of reactive metabolites, genotoxicity, and chemical and photo-mutagenicity assays. Written for the Methods in Pharmacology and Toxicology sequence, chapters comprise introductions to their respective issues, lists of the required fabrics and reagents, step by step, with no trouble reproducible protocols, and pointers on troubleshooting and warding off recognized pitfalls. specialist authors have constructed and applied those in vitro assays to accomplish “drug-like” features as well as efficacy houses and stable security profiles of drug candidates.

Comprehensive and updated, Optimization in Drug Discovery: In Vitro equipment, moment Edition goals to steer researchers down the tough route to winning drug discovery and development.

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Completely revised and up-to-date, Optimization in Drug Discovery: In Vitro equipment, moment version offers a large spectrum of in vitro assays together with formula, plasma binding, absorption and permeability, cytochrome P450 (CYP) and UDP-glucuronosyltransferases (UGT) metabolism, CYP inhibition and induction, drug transporters, drug-drug interactions through review of reactive metabolites, genotoxicity, and chemical and photo-mutagenicity assays.

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The %CV was typically 15 % or less for all compounds except for Itraconazole which was about 20 %. The experiment was repeated if there was more than 30 % average deviation between the replicates. 2 (9 %) Since all the test compounds were available commercially in high purity, we wanted to see how real drug candidate compounds synthesized in small batches would behave in the assay. We were ­particularly interested in seeing if the buffering capacity of the citrate (pH 4) (Table 3) and Tris (pH 8) (Table 4) would be maintained.

J Pharm Sci 86(2): Tech 35(8):1001–1010 147–162 Chapter 2 Assessment of Drug Plasma Protein Binding in Drug Discovery Dennis Kalamaridis and Nayan Patel Abstract Determination of plasma protein binding is important in drug discovery and development for developing PK-PD relationships, and projecting clinical doses because the free drug concentration at the site of action is responsible for the pharmacological activity. This chapter will describe various methods commonly used for the in vitro determination of plasma protein binding including classical equilibrium dialysis (CED), rapid equilibrium dialysis (RED), and ultrafiltration Nonspecific binding and mass balance methods used to recover or prevent the loss of compound due to binding to the wall and/or membrane of the apparatus will be briefly discussed as well.

When using radiolabeled materials (3H or 14C-labeled) the method of analysis of both plasma and buffer samples recovered from each half-cell will be liquid scintillation spectrometry. Usually 50–100 μL of each matrix is added to scintillation vials containing cocktail (Ultima Gold®, Perkin Elmer, Shelton, CT) and each sample collected is counted in duplicate in a liquid scintillation analyzer (Packard TriCarb 3100TR, Perkin Elmer, Shelton, CT). 5 %) as larger volumes can be counted in order to obtain sufficient counts for the calculation of fu.

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